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Developing a novel and simple biosensor for Cystatin C as a fascinating marker of glomerular filtration ratewith DNase I-aided recycling amplification strategy

发布时间:2022-07-11 文章来源: 深圳市药品检验研究院
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  Wang B,Yu XA,Yin G,Wang J,Jin YB,Wang TJ

  (Shenzhen Inst Drug Control, NMPA Key Lab Bioequivalence Res Gener Drug Evalua)

  Abstract:Cystatin C (Cys C) has been proposed as a fascinating glomerular filtration rate (GFR) marker for early detection of acute kidney injury and chronic kidney disease. However, most of traditional methods for Cys C detection are immunoassays, which was tedious to perform and unfriendly for economics. In this work, a novel and simple biosensor for the sensitive measurement of Cys C via DNase I-aided recycling amplification strategy was successfully constructed based on the graphene oxide (GO) and fluorophore-labelled aptamer, which can be used to the early prediction of kidney injury. The fluorescence of fluorophore-labelled aptamer was quenched by GO based on the Fluorescence Resonance Energy Transfer (FRET) and recovered with the existence of Cys C. In addition, the DNase I enzyme would digest the fluorophore-labelled aptamer and dissociate the Cys C to launch the next reaction, resulting in an increase of signal amplification. Hence, the limit of detection is found to be 0.16 ng mL(-1), which is almost 3 times lower than that without DNase I. Consequently, the developed biosensor offers a novel approach towards simple and rapid detection of Cys C based on the integration of GO and aptamer. Conceivably, this strategy holds a wide scope in the application of numerous other analytes if corresponding aptamers are available. (C) 2021 Elsevier B.V. All rights reserved.

  Keywords:

  Biosensor;Cystatin C;Kidney injury;Recycling amplification strategy

  该论文发表于《JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS》2021年第203卷


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